Icelandic Horse Connection

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Published recently in the Veterinary Immunology and Immunopathology, is a study on IBDH (which stands for "insect bite dermal hypersensitivity") in Icelandic Horses.

Production of monoclonal antibodies specific for native equine IgE and their application to monitor total serum IgE responses in Icelandicnext term and non-previous termIcelandic horsesnext term with insect bite dermal hypersensitivity

A. Douglas Wilsona, Corresponding Author Contact Information, E-mail The Corresponding Author, Lisa Harwoodb, Sigurbjörg Torsteinsdottirc and Eliane Martib

aDivision of Pathology Infection and Immunity, University of Bristol, School of Clinical Veterinary Science, Langford House, Langford BS40 5DU, UK

bDivision of Clinical Research, Department of Clinical Veterinary Medicine, Bremgartenstr. 109a, P.O. Box 3001, Berne, Switzerland

cInstitute for Experimental Pathology, University of Iceland, Keldur v/Vesturlandsveg, 112 Reykjavik, Iceland

Received 25 November 2005; revised 16 January 2006; accepted 15 February 2006. Available online 29 March 2006.

Abstract Immunoglobulin E forms a minor component of serum antibody in mammals. In tissues IgE is bound by Fcvar epsilonRI receptors on the surface of mast cells and mediates their release of inflammatory substances in response to antigen. IgE and mast cells have a central role in immunity to parasites and the pathogenesis of allergic diseases in previous termhorsesnext term and other mammals. This paper describes the production of several novel monoclonal antibodies that detect native equine IgE in immunohistology, ELISA and Western blotting. An antigen capture ELISA to quantify equine IgE in serum has been developed using two of these antibodies. The mean serum IgE concentration of a group of 122 adult previous termhorsesnext term was 23,523 ng/ml with a range of 425–82,610 ng/ml. Total serum IgE of healthy previous termhorsesnext term was compared with that of previous termhorsesnext term with insect bite dermal hypersensitivity (IBDH) an allergic reaction to the bites of blood feeding insects of Culicoides or Simulium spp. IBDH does not occur in Iceland where Culicoides spp. are absent, but following importation into mainland Europe native previous termIcelandic horsesnext term have an exceptionally high incidence of this condition. In the present study previous termIcelandic horsesnext term with IBDH had significantly higher total IgE than healthy previous termIcelandic horsenext term controls (P < 0.05). By contrast in previous termhorsesnext term of other breeds the difference in total serum IgE between those affected with IBDH and healthy controls was not statistically significant. Total serum IgE was also monitored in a cohort of previous termIcelandic horsesnext term prior to import into Switzerland and for a period of 3 years thereafter. High levels of serum IgE were present in all previous termhorsesnext term at the start of the study but dropped in the first year after import. Thereafter the total serum IgE remained low in previous termIcelandic horsesnext term that remained healthy but rose significantly (P < 0.05) in those that developed IBDH. These results support the conclusion that IBDH is a type I hypersensitivity response to insect allergens but indicate that IBDH in previous termIcelandic horsesnext term may have a different pathogenesis from the same condition in other breeds.
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